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KMID : 1149220100180020241
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Korean Journal of Oriental Medical Prescription
2010 Volume.18 No. 2 p.241 ~ p.249
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Antiinflammatory Effect of Bioactive Compounds from Geranium nepalense (Notes)
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Kim Bo-Mi
Kim Eui-Sung
Lee Young-Hang
Yu Byung-Soo
Chai Kyu-Yun
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Abstract
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The Geranium nepalense has been used traditionally for treatment of various diseases. However, the molecular studies on the effect of Geranium nepalense have not been carried out. In the present study, Quercetin, quercitrin, and afzelin were isolated from the methanol extract of Geranium nepalense were tested for their anti-inflammatory effect. The anti-inflammatory effect of the compounds was studied in lipopolysaccharide(lps)-treated mouse macrophage cells, RAW 264.7. RAW 264.7 cells were pre-incubated with isolated compounds(0, 5, 10, 20, 40, ) for 4h and treated with lps for 18h, and then the anti-inflammatory effects of compounds were determined. The results are as follows: Quercetin at various concentration inhibited the viability of Raw 264.7 from 7% to 45%, quercitrin from 25% to 80%, and afzelin from 13% to 52%. Isolated compounds showed a significant decrease in iNOS (inducible nitric oxide synthase) and COX-2 (cyclooxygenase-2). These results suggest that these compounds can be used as stable anti-inflammatory materials.
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KEYWORD
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Quercetin, quercitrin, afzelin, Geranium nepalense, nitric oxide, prostaglandin E2, inducible nitric oxide synthase, cyclooxygenase-2
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